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Sciences in Cold and Arid Regions ›› 2022, Vol. 14 ›› Issue (2): 109-119.doi: 10.3724/SP.J.1226.2022.21054.

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Diversity and composition of culturable fungi in Horqin Sandy Land

ShaoKun Wang1,2,3(),XueYong Zhao1,2,3,Hao Qu1,3,Jie Lian2,3,Fei Wang1,2,FengHua Ding4   

  1. 1.Urat Desert-grassland Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Science, Lanzhou, Gansu 730000, China
    2.Naiman Desertification Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Science, Lanzhou, Gansu 730000, China
    3.Key Laboratory of Stress Physiology and Ecology, Gansu Province, Lanzhou, Gansu 730000, China
    4.Lishui University, Lishui, Zhejiang 323000, China
  • Received:2021-07-03 Accepted:2021-11-07 Online:2022-04-30 Published:2022-04-25
  • Contact: ShaoKun Wang E-mail:wangsk@lzb.ac.cn
  • Supported by:
    the National Nature Science Foundation of China(41771117);the China National Key Research and Development Plan(2017FY100200);the Second Tibetan Plateau Scientific Expedition and Research program(2019QZKK0305);the Key Research and Development project of Zhejiang Province(2018C02031)

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Abstract:

Soil fungi play a key role in soil functional performance and ecological restoration. To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land, China, mobile dune, semi-fixed dune, fixed dune and sandy grassland were selected to investigate the soil fungal diversity using a traditional culture-dependent approach. ITS sequencing was applied to identify the fungal strains. The counts of culturable fungi increased significantly from mobile dune to sandy grassland along the gradient of sandy land restoration. The Shannon-Wiener, Simpson and Evenness indices of culturable fungi ranged from 1.26-1.71, 0.22-0.37 and 0.83-0.87, respectively. A total of 27 fungal strains were isolated using dilution plate cultural technique. The 27 fungal isolates were clustered into three groups: Ascomycota, Basidiomycota and Mucoromycota at phylum level, indicating that Ascomycota was the dominant fungal phylum (88.9% of the total). The isolated fungi were grouped into 3 phyla, 5 classes, 6 orders, 11 families and 13 genera. The results show that culturable fungi were diverse in sandy land soils and fungal isolates have potential function in lipid turnover, cellulose degradation and ethanol, glucose and fatty acid production. Future studies should be carried out to explore their ecological and biological function for degraded sandy land restoration.

Key words: Culturable fungi, fungal diversity, ITS sequences, phylogenetic analysis, Horqin Sandy Land

Figure 1

Study area of Horqin Sandy Land"

Figure 2

Culturable fungal abundance in the fourhabitats of Horqin Sandy Land"

Table 1

Identification of DNA sequences and their taxonomic affiliations in NCBI using BLAST"

Strain No.G+CLength (bp)Accession number in GenBankMost similar stainSimilarity
S0159.79%470JN676110.1Aspergillus japonicus isolate B499.40%
S0249.11%448MK764999.1Fusarium equiseti isolate NX2-199.79%
S0351.09%460MH517365.1Fusarium brachygibbosum isolate AV799.40%
S0460.71%476MK720828.1Penicillium ochrochloron strain KD-F198.64%
S0551.40%465MK696907.1Fusarium tricinctum isolate C14-11B99.01%
S0655.75%522KC576722.1Trichoderma koningiopsis strain CEN71899.82%
S0756.99%479DQ339549.1Penicillium griseofulvum strain NRRL 3523100.00%
S0837.57%535MH854979.1Mucor circinelloides strain CBS 202.2899.46%
S0961.05%493GU966506.1Penicillium javanicum strain WTS2799.07%
S1049.69%491FJ426391.1Fusarium chlamydosporum isolate DB-L3999.80%
S1154.13%484MT251175.1Fusarium falciforme strain DTO 422-H899.80%
S1257.68%482EF669945.1Neosartorya aureola isolate NRRL 2064399.41%
S1345.07%497KF887142.1Alternaria tenuissima strain S2499.79%
S1454.58%469KY310738.1Humicola fuscoatra isolate 10500299.79%
S1560.04%473MK450677.1Penicillium caperatum strain CMV001H199.59%
S1655.20%558MK871030.1Trichoderma sp. isolate SDAS20416698.97%
S1748.33%480MN959998.1Fusarium oxysporum isolate 1599.60%
S1846.87%463MW723759.1Didymella sp. isolate 63JAN97.80%
S1947.07%444KY318503.1Coniothyrium aleuritis isolate 4297.98%
S2058.81%505KC790523.1Neosartorya sp. 8-13c99.62%
S2148.97%290MK299139.1Fusarium brachygibbosum isolate LWU_4090.30%
S2247.65%447MN833380.1Fusarium oxysporum isolate 83#99.59%
S2357.73%440MF574327.1Penicillium javanicum isolate Y9_ITS1F99.77%
S2452.12%449MH001945.1Myrothecium lachastrae isolate 13-191397.62%
S2535.67%485JQ683240.1Cunninghamella echinulata isolate A-33293.74%
S2649.10%446MH173818.1Fusarium chlamydosporum isolate DOBPGPF V3798.12%
S2742.02%545MN759032.1Naganishia albida isolate en11998.96%

Figure 3

Culturable fungal diversity indices in the four habitats of Horqin Sandy Land"

Figure 4

Phylogenetic Maximum Likelihood (ML) tree of the 27 fungal isolates and their most related strains in GenBank"

Figure 5

The proportion of different taxa at phylum, class, order, family and genus levels"

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