Sciences in Cold and Arid Regions ›› 2016, Vol. 8 ›› Issue (3): 205-211.doi: 10.3724/SP.J.1226.2016.00205

• ARTICLES • Previous Articles    

Proline and soluble sugars accumulation in three pepper species (Capsicum spp) in response to water stress imposed at different stages of growth

Gideon O. Okunlola, Richard O. Akinwale, Adekunle A. Adelusi   

  1. 1. Department of Biological Sciences, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Osun State, Nigeria;
    2. Department of Crop Production and Protection, Faculty of Agriculture, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria;
    3. Department of Botany, Faculty of Science, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria
  • Received:2015-11-30 Revised:2016-03-25 Published:2018-11-23
  • Contact: Gideon O.Okunlola,Department of Biological Sciences,Faculty of Basic and Applied Sciences,Osun State University,Osogbo,Osun State,Nigeria.Tel:+2348033870696;

Abstract: Drought is a major production constraint for major fruits and vegetable crops in the tropics.This study was conducted to investigate the effect of limited water supply at three growth stages (vegetative,flowering and fruiting) on the accumulation of proline and soluble sugars in three pepper species.Seeds of the three pepper species,Capsicum chinense Jacq.,C.annuum L.and C.frutescens L.were raised in a nursery and the seedlings were transplanted into seventy two plastic pots arranged in a randomized complete block design with three replicates,25 days after planting.Four water treatments,200 mL of water supplied twice daily (W1),once in every three days (W2),once in every five days (W3),and zero water supplied throughout growing period (W0) were imposed at three vegetative,flowering,and fruiting growth stages.Data were collected on relative water content,free proline and total soluble sugar.Data collected were subjected to analysis of variance and means were separated using Duncan's multiple range test.Results show that the concentration of proline and soluble sugar in leaves of the three pepper species were found to be remarkable at the different stages of growth in the stressed plants.

Key words: growth, osmolites, pepper, proline, stress, sugar

Arabzadeh N, 2012. The effect of drought stress on soluble carbohydrates (Sugars) in two species of Haloxylon persicum and Haloxylon aphyllum. Asian Journal of Plant Science, 11(1):44-51.
Aslam M, Khan IA, Saleem M, et al., 2006. Assessment of water stress tolerance in different maize accessions at germination and early growth stage. Pakistan Journal of Botany, 38:1571-1579.
Bajji M, Lutts S, Kinet JM, 2001. Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science, 160:669-681.
Bates LS, Waldren RP, Teare ID, 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39:205-207.
Bayoumi TY, Eid MH, Metwali EM, 2008. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology, 7:2341-2352.
Blum A, 1996. Crop response to drought and the interpretation of adaptation. Journal of Plant Growth Regulation, 20(2):135-148.
Caballero JI, Verduzco CV, Galan J, et al., 2005. Proline accumulation as a symptom of drought stress in maize:A tissue differentiation requirement. Journal of Experimental Botany, 39:889-897.
Cattivelli L, Rizza F, Badeck FW, et al., 2008. Drought tolerance improvement in crop plants:An integrated view from breeding to genomics. Field Crops Research, 105(1-2):1-14.
Claussen W, 2005. Proline as a measure of stress in tomato plants.Plant Science, 168:241-248.
Clifford SC, Arndt SK, Corlett JE, et al., 1998. The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.).Journal of Experimental Botany, 49:967-977.
Cobley LS, Steele WM, 1976. Introduction to the Botany of Tropical Crops. 2nd Edition. London:Longman, pp. 371.
Delauney AJ, Verma DPS, 1993. Proline biosynthesis and osmoregulation in plants. The Plant Journal, 4:215-223.
Dubois M, Gilles KA, Hamilton JK, et al., 1956. Colorimetric method for determination of sugar and related substances. Analytical Chemistry, 28(3):350-356.
FAOSTAT, 2001. Hot pepper production in sub-Saharan Africa. A Report. Journal of Crop Science. Food and Agriculture Organization, pp. 23.
Grand K, Kreyling J, Dienstbach LFH, et al., 2014. Water stress due to increased intra-annual precipitation variability reduced forage yield but raised forage quality of a temperate grassland.Agriculture, Ecosystems and Environment, 186:11-22.
Greenleaf WH, 1986. Breeding vegetable crops, Chapter 3. Pepper breeding. In:Basset MJ (ed.). The AVI Publishing Company Inc. Westport, Connecticut, pp. 67-134.
Grubben GJH, El Tahir IM, 2004. Capsicum annuum L. In:Grubben GJH, Denton OA (eds.). PROTA 2:Vegetables/Légumes.[CD-Rom]. PROTA, W ageningen, The Netherlands.
Gzik A, 1996. Accumulation of proline and pattern of D-amino acids in sugar beet plants in response to osmotic, water and salt stress. Journal of Environment and Experimental Botany, 36:29-38.
Izanloo A, Condon AG, Langridge P, et al., 2008. Different mechanisms of adaptation to cyclic water stress in two South Australian bread wheat cultivars. Journal of Experimental Botany, 59(12):3327-3346.
Jajarmi V, 2009. Effect of water stress on germination indices in seven wheat cultivar. World Academy of Science, Engineering and Technology, 49:105-106.
Jones MM, Turner NC, 1980. Accumulation of solutes in leaves of sorghum and sunflower in response to water deficits. Australian Journal of Plant Physiology, 7:193-205.
Kishor PBK, Sangama S, Amrutha RN, et al., 2005. Regulation of proline biosynthesis degradation, uptake and transport in higher plants:its implications in plant growth and abiotic stress tolerance. Current Science, 88:424-438.
Lipiec J, Doussan C, Nosalewicz A, et al., 2013. Effect of drought and heat stresses on plant growth and yield:A review. International Agrophysics, 27:463-477.
Maggio A, Miyazaki S, Veronese P, et al., 2002. Does proline accumulation play an active role in stress-induced growth reduction? Plant Journal, 31:699-712.
Passioura JB, 2007. The drought environment:physical, biological and agricultural perspectives. Journal of Experimental Botany, 58:113-117.
Rhodes D, Samaras Y, 1994. Genetic control of osmoregulation in plants. In:Stronge K (ed.). Cellular and Molecular Physiology of Cell Volume Regulation. Boca Raton:CRC Press, pp. 347-361.
Sanusi MM, Ayinde IA, 2013. Profitability of pepper production in derived savannah zone of Ogun State, Nigeria. IJAFS 4, 2:401-410.
Saruhan N, Terzi R, Kadioglu A, 2006. The effects of exogenous polyamines on some biochemical changes during drought stress in Ctenanthe setosa. Acta Biologica Hungarica, 57(2):221-229.
Sharma SS, Dietz KJ, 2006. The significance of amino acids and amino-acid derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany, 57:711-726.
Siddique MRB, Hamid A, Islam MS, 2000. Drought stress effects on water relations of wheat. Botanical Bulletin of Academia Sinica, 41(1):35-39.
Singh DK, Sale PWG, Pallaghy CK, et al., 2000. Role of proline and leaf expansion rate in the recovery of stressed white clover leaves with increased phosphorus concentration. New Phytologist, 146:261-269.
Umezawa T, Fujita M, Fujita Y, et al., 2006. Engineering drought tolerance in plants:discovering and tailoring genes to unlock the future. Current Opinion in Biotechnology, 17:113-122.
Wang WX, Vinocur P, Altman A, 2003. Plant responses to drought, salinity and extreme temperatures:towards genetic engineering for stress tolerance. Planta, 218:1-14.
Watanabe S, Kojima K, Ide Y, et al., 2000. Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell, Tissue and Organ Culture, 63:199-206.
Xonostle-Cazares B, Ramirez-Ortega FA, Flores-Elenes L, et al., 2010. Drought tolerance in crop plants. American Journal of Plant Physiology, 5:241-256.
[1] YuTing Liang,XingDong He,JianTan Guo,HongJuan Jing. Effects of N:P ratio of Artemisia ordosica on growth influenced by soil calcium carbonate [J]. Sciences in Cold and Arid Regions, 2018, 10(4): 333-339.
[2] TingTing Xie, PeiXi Su, WenZhi Zhao, LiShan Shan. Cluster planting impact on cotton growth, yield and biomass accumulation in an arid region oasis [J]. Sciences in Cold and Arid Regions, 2018, 10(3): 271-278.
[3] QingZhi Wang, JianKun Liu, JianHong Fang, AnHua Xu. Direct shear tests of coarse-grained fillings from high-speed railway subgrade in cold regions [J]. Sciences in Cold and Arid Regions, 2017, 9(3): 236-242.
[4] JianWei Wang, HaiPeng Li, Lei Song, Shuai Dou, XinLei Na. Effect of cryostructures on the uniaxial compressive strength of frozen clay [J]. Sciences in Cold and Arid Regions, 2017, 9(3): 267-272.
[5] Alexey F. Kolos, Anastasia A. Konon. Test results of railway ballast for bearing capacity calculations [J]. Sciences in Cold and Arid Regions, 2017, 9(3): 312-316.
[6] Evgeny S. Ashpiz, Tatyana S. Vavrinyuk. Strengthening long-term embankments maintained on permafrost soils [J]. Sciences in Cold and Arid Regions, 2017, 9(3): 317-320.
[7] Ezekiel Dare Olowolaju, Adekunle Ajayi Adelusi. Photosynthetic pigments accumulation and some growth indices of cowpea, maize and tomato in response to interspecific and intraspecific competition stress [J]. Sciences in Cold and Arid Regions, 2017, 9(2): 120-126.
[8] Fang Wang, HongLang Xiao, XiaoMei Peng, Shang Li. Effects of salt and alkali stress on Reaumuria soongorica germination [J]. Sciences in Cold and Arid Regions, 2017, 9(2): 158-166.
[9] XiaoMei Peng, ShengChun Xiao, GuoDong Cheng, QuanYan Tian, HongLang Xiao. Microcoring and dendrometer-detected intra-annual wood formation of Populus euphratica in the Ejina Oasis,northwestern China [J]. Sciences in Cold and Arid Regions, 2017, 9(1): 54-66.
Full text



No Suggested Reading articles found!