Sciences in Cold and Arid Regions ›› 2021, Vol. 13 ›› Issue (4): 326-336.doi: 10.3724/SP.J.1226.2021.20025.

Previous Articles    

Ecophysiological responses to drought stress in Populus euphratica

ChunYan Zhao1,2,JianHua Si1,2(),Qi Feng1,2,TengFei Yu1,2,Huan Luo1,3,Jie Qin1,3   

  1. 1.Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
    2.Key Laboratory of Ecohydrology of Inland River Basin, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
    3.University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2020-04-22 Accepted:2020-10-21 Online:2021-08-31 Published:2021-08-19
  • Contact: JianHua Si E-mail:jianhuas@lzb.ac.cn

Abstract:

Ecophysiological responses to drought stress of Populus euphratica in Alashan Desert Eco-hydrology Experimental Research Station were investigated. Results show that under mild and moderate drought stress, stomatal length, aperture, area and density is likely to decrease in the early days, but afterwards this is likely to recovery with treatment over the passage of treatment time. Under severe drought stress, these properties appear to decline continuously. However, after 45 days of drought-stress treatment, the decline is not as noticeable as before, indicating that Populus euphratica could possibly reduce water evaporation by shutting down the stoma, leading to an improvement in its water use efficiency with better survival under drought stress conditions. The leaf area first decreases, and then increases under mild and moderate drought stress conditions, with the average values under different degree of stress found to be approximately 129.52, 120.08, 116.63 and 107.28 cm2, respectively. Under moderate stress conditions, the leaf water potential appears to show a continuous decline where the average values under different degree of stress are found to be -1.27, -1.85, -4.29 and -4.80 MPa, respectively. In terms of proline content, the results demonstrate that this factor appears to increase significantly under moderate and severe drought stress conditions. Especially under severe drought stress condition, the content is found to be more than 700 μg/g. Ranging over average values of 14.64 and 15.90 nmol/g under moderate and severe drought stress, respectively, Malondialdehyde content is found to increase quite rapidly under moderate and severe drought stress conditions at first, which then appears to decrease gradually with the treatment over time.

Key words: stomatal morphology, drought stress, Malondialdehyde, proline, Populus euphratica

Figure 1

An example for stomata of P. euphratica under different degrees of drought stress (ND = no drought stress; LD = mild drought stress; MD = moderate drought stress; SD = severe drought stress)"

Figure 2

Temporal changes in environmental variables during the experimental phase in 2016 (daily means ± standard deviation)"

Figure 3

Changes in P. euphratica stomatal, aperture, area and density under different degrees of drought stress (ND = no drought stress; LD = mild drought stress; MD = moderate drought stress; SD = severe drought stress)"

Figure 4

Changes in leaf area and leaf water potential of P. euphratica during the experimental period (ND = no drought stress; LD = mild drought stress; MD = moderate drought stress; SD = severe drought stress)"

Figure 5

Change in malondialdehyde (MDA) and proline content of P. euphratica during the experimental period (ND = no drought stress; LD = mild drought stress; MD = moderate drought stress; SD = severe drought stress)"

Figure 6

The response relationship between soil water content and stomatal length, aperture, area and density, leaf area, leaf water potential, proline content and MDA content of P. euphratica (ND = no drought stress; LD = mild drought stress; MD = moderate drought stress; SD = severe drought stress)"

Anyia AO, Herzog H, 2004. Water-use efficiency, leaf area and leaf gas exchange of cowpeas under mid-season drought. European Journal of Agronomy, 20: 327-339. DOI: 10.1016/S1161-0301(03)00038-8.
doi: 10.1016/S1161-0301(03)00038-8
Bailly C, Benamar A, Corbineau F, et al., 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Plant Physiology, 97: 104-110. DOI: 10.1111/j.1399-3054.1996.tb00485.x.
doi: 10.1111/j.1399-3054.1996.tb00485.x
Barnabas B, Jager K, Feher A, 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant Cell and Environment, 31: 11-38. DOI: 10.1111/j.1365-3040. 2007.01727.x.
doi: 10.1111/j.1365-3040. 2007.01727.x
Berova M, Stoilova T, Kuzmova K, et al., 2012. Changes in the leaf gas exchange, leaf water potential and seed yield of cowpea plants (Vigna unguiculata L.) under soil drought conditions. Agricultural Sciences, 29-34.
Buckley TN, 2005. The control of stomata by water balance. New Phytologist, 168: 275-292. DOI: 10.1111/j.1469-8137. 2005.01543.x.
doi: 10.1111/j.1469-8137. 2005.01543.x
Bates LS, Waldren RP, Teare ID,1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205-207. DOI: 10.1007/BF00018060.
doi: 10.1007/BF00018060
Dasgupta P, Das BS, Sen SK, 2015. Soil water potential and recoverable water stress in drought tolerant and susceptible rice varieties. Agricultural Water Management, 152: 110-118. DOI: 10.1016/j.agwat.2014.12.013.
doi: 10.1016/j.agwat.2014.12.013
Devireddy AR, Arbogast J, Mittler R, 2019. Coordinated and rapid whole‐plant systemic stomatal responses. New Phytologist, 225: 1. DOI: 10.1111/nph.16143.
doi: 10.1111/nph.16143
Esmailpour A, Labeke MCV, Samson R, et al., 2016.Variation of relative water content, water use efficiency and stomatal density during drought stress and subsequent recovery in Pistachio Cultivars (Pistacia vera L.). The International Horticultural Congress, 1109: 113-119. DOI: 10. 17660/ActaHortic.2016.1109.18.
doi: 10. 17660/ActaHortic.2016.1109.18
Franks PJ, Farquhar GD, 2001. The effect of exogenous abscisic acid on stomatal development, stomatal mechanics, and leaf gas exchange in Tradescantia virginiana. Plant Physiology, 125: 935-942. DOI: 10.1104/pp.125.2.935.
doi: 10.1104/pp.125.2.935
Gomez-Del-Campo M, Ruiz C, Baeza P, et al., 2003. Drought adaptation strategies of four grapevine cultivars (Vitis vinifera L.): modification of the properties of the leaf area. Journal International Des Sciences De La Vigne Et Du Vin, 37: 131-143. DOI: 10.1080/00397910802632589.
doi: 10.1080/00397910802632589
Guo YY, Yu HY, Yang MM, et al., 2018. Effect of drought stress on lipid peroxidation,osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedlin. Russian Journal of Plant Physiology, 65: 244-250. DOI: 10.1134/S1021443718020127.
doi: 10.1134/S1021443718020127
Grzesiak MT, Grzesiak S, Skoczowski A, 2006. Changes of leaf water potential and gas exchange during and after drought in triticale and maize genotypes differing in drought tolerance. Photosynthetica, 44: 561-568. DOI: 10. 1007/s11099-006-0072-z.
doi: 10. 1007/s11099-006-0072-z
Hanif S, Saleem MF, Sarwar M, 2020. Biochemically triggered heat and drought stress tolerance in rice by proline application. Journal of Plant Growth Regulation, 2020: 20. DOI: 10.1007/s00344-020-10095-3.
doi: 10.1007/s00344-020-10095-3
Heidari Y, Moaveni P, 2009. Study of drought stress on ABA accumulation and proline among in different genotypes forage corn. Research Journal of Biological Sciences, 4: 1121-1124. DOI: 1815-8846.
doi: 1815-8846
Iwai S, Shimomura N, Nakashima A, et al., 2003. New fava bean guard cell signaling mutant impaired in ABA-induced stomatal closure. Plant and Cell Physiology, 44: 909-913. DOI: 10.1093/pcp/pcg116.
doi: 10.1093/pcp/pcg116
Iqbal MJ, Maqsood Y, Abdin ZU, et al., 2016. SSR markers associated with proline in drought tolerant wheat germplasm. Applied Biochemistry and Biotechnology, 178: 1-11. DOI: 10.1007/s12010-015-1927-1.
doi: 10.1007/s12010-015-1927-1
Inamullah, Isoda A, 2005. Adaptive reponses of soybean and cotton to water stress I.Transpiration changes in relation to stomatalarea and stomatal conductance. Plant Production Science, 8: 16-26. DOI: 10.1626/pps.8.16.
doi: 10.1626/pps.8.16
Jensen CR, Mogensen VO, Mortensen G, et al., 1996. Leaf photosynthesis and drought adaptation in field-grown Oilseed Rape (Brassica napus L.). Functional Plant Biology, 23: 631-644. DOI: 10.1071/PP9960631.
doi: 10.1071/PP9960631
Jongdee B, Fukai S, Cooper M, 2002. Leaf water potential and osmotic adjustment as physiological traits to improve drought tolerance in rice. Field Crops Research, 76: 153-163. DOI: 10.1016/S0378-4290(02)00036-9.
doi: 10.1016/S0378-4290(02)00036-9
Johnson DM, Wortemann R, McCulloh KA J, et al., 2016. A test of the hydraulic vulnerability segmentation Hypothesis in angiosperm and conifer tree species. Plant Physiology, 36: 983-993. DOI: 10.1093/treephys/tpw031.
doi: 10.1093/treephys/tpw031
Li F, Kang S, Zhang J, 2004. Interactive effects of elevated CO2, nitrogen and drought on leaf area, stomatal conductance, and evapotranspiration of wheat. Agricultural Water Management, 67: 221-233. DOI: 10.1016/j.agwat.2004.01.005.
doi: 10.1016/j.agwat.2004.01.005
Li HS, 2000. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, pp. 260-263.
Liu CC, Liu YG, Guo K, et al., 2016. Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in Karst habitats of southwestern China. Environmental & Experimental Botany, 71: 174-183. DOI: 10.1016/j.envexpbot.2010.11.012.
doi: 10.1016/j.envexpbot.2010.11.012
Lum MS, Hanafi MM, Rafii YM, et al., 2014. Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. The Journal of Animal and Plant Sciences, 25: 1487-1493.
Massonnet C, Dauzat M, Bédiée A, et al., 2015. Individual leaf area of early flowering Arabidopsis Genotypes is more affected by drought than late flowering ones:A multi-scale analysis in 35 genetically modified lines. American Journal of Plant Sciences, 6: 955-971. DOI: 10.4236/ajps. 2015. 67102.
doi: 10.4236/ajps. 2015. 67102
Maréchaux I, Bartlett MK, Sack L, 2015. Drought tolerance as predicted by leaf water potential at turgor loss point varies strongly across species within an Amazonian forest. Functional Ecology, 29: 1268-1277. DOI: 10.1111/1365-2435. 12452.
doi: 10.1111/1365-2435. 12452
Man D, Bao YX, Han LB, et al., 2011. Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. Hortscience A Publication of the American Society for Horticultural Science, 46: 1027-1032. DOI: 10. 21273/HORTSCI.46.7.1027.
doi: 10. 21273/HORTSCI.46.7.1027
Pan X, 2013. Tall fescue performance and protein alteration during drought stress. Dissertations and Theses, 465-473. DOI: http://hdl.handle.net/11244/9590.
doi: http://hdl.handle.net/11244/9590
Pradhan GP, Prasad PVV, Fritz AK, et al., 2012. Effects of drought and high temperature stress on synthetic hexaploid wheat. Functional Plant Biology, 39: 190-198. DOI: 10. 1071/FP11245.
doi: 10. 1071/FP11245
Rood SB, Patiño S, Coombs K, et al., 2000. Branch sacrifice cavitation-associated drought adaptation of riparian cotton woods. Trees, 14: 248-257. DOI: 10.1007/s004680050010.
doi: 10.1007/s004680050010
Singh TN, Aspinall D, Paleg LG,1972. Proline accumulation and varietal adaptability to drought in barley: a potential metabolic measure of drought resistance. Nature New Biology, 12: 188-190. DOI: 10.1038/newbio236188a0.
doi: 10.1038/newbio236188a0
Si JH, Chang ZQ, Su YH, et al., 2008. Stomatal conductance characteristics of Populus euphratica leaves and response to environmental factors in the extreme arid region. Acta Botanica Boreali-Occidenalia Sinica, 28: 125-130. DOI: 10.1109/LAWP.2012.2236878.
doi: 10.1109/LAWP.2012.2236878
Soleimanzadeh H, Habibi D, Ardakani MR, et al., 2010. Effect of potassium levels on antioxidant enzymes and malondialdehyde content under drought stress in sunflower (Helianthus annuus L.). American Journal of Agricultural & Biological Science, 5: 56-61. DOI: 10.3844/ajabssp.2010. 56.61.
doi: 10.3844/ajabssp.2010. 56.61
Yousifi N, Slama I, Ghnaya T, et al., 2010. Effects of water deficit stress on growth, water relations and osmolyte accumulation in Medicago truncatula and M. laciniata populations. C R Biol, 333: 205-213. DOI: 10.1016/j.crvi. 2009.12.010.
doi: 10.1016/j.crvi. 2009.12.010
Zhai Y, Shao S, Sha W, et al., 2017. Over expression of soybean GmERF9 enhances the tolerance to drought and cold in the transgenic tobacco. Plant Cell, Tissue and Organ Culture, 128: 607-618. DOI: 10.1007/s11240-016-1137-8.
doi: 10.1007/s11240-016-1137-8
Zhang RH, Guo DW, Zhang XH, et al., 2012. Effects of drought stress on physiological characteristics and dry matter production in maize silking stage. Acta Agronomica Sinica, 38: 1884-1890. DOI: 10.3724/SP.J.1006.2012. 01884.
doi: 10.3724/SP.J.1006.2012. 01884
[1] ZongQiang Chang, Hua Tao, Qiang Zhu. Seasonal characteristics of chlorophyll fluorescence kinetics of heteromorphic leaves in Populus Euphratica [J]. Sciences in Cold and Arid Regions, 2018, 10(2): 169-179.
[2] Gideon O. Okunlola, Richard O. Akinwale, Adekunle A. Adelusi. Proline and soluble sugars accumulation in three pepper species (Capsicum spp) in response to water stress imposed at different stages of growth [J]. Sciences in Cold and Arid Regions, 2016, 8(3): 205-211.
[3] XiaMei Yao, Jing Ma, Jing Ji, Chun Ou, WenQiang Gao. Effect of exogenous application of salicylic acid on the drought stress responses of Gardenia jasminoides [J]. Sciences in Cold and Arid Regions, 2016, 8(1): 54-64.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!