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Sciences in Cold and Arid Regions ›› 2019, Vol. 11 ›› Issue (2): 139–149.doi: 10.3724/SP.J.1226.2019.00139.

• • 上一篇    

  

  • 收稿日期:2018-12-24 接受日期:2019-02-28 出版日期:2019-04-01 发布日期:2019-04-29

Shifts in community structure and function of ammonia-oxidizing archaea in biological soil crusts along a revegetation chronosequence in the Tengger Desert

LiNa Zhao1,2,4,XinRong Li1,2,ShiWei Yuan3,4,YuBing Liu1,2()   

  1. 1. Shapotou Desert Research and Experiment Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
    2. Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
    3. Key Laboratory of Remote Sensing of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
    4. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2018-12-24 Accepted:2019-02-28 Online:2019-04-01 Published:2019-04-29
  • Contact: YuBing Liu E-mail:liuyb@lzb.ac.cn
  • About author:Professor YuBing Liu, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences. No. 320, West Donggang Road, Lanzhou, Gansu 730000, China. Tel: +86-931-4967199; E-mail: liuyb@lzb.ac.cn

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Abstract:

Metagenomic studies have demonstrated the existence of ammonia-oxidizing archaea (AOA) and revealed they are responsible for ammoxidation in some extreme environments. However, the changes in compositional structure and ammonia-oxidation capacity of AOA communities in biological soil crusts (BSCs) of desert ecosystems remain poorly understood. Here, we utilized Illumina MiSeq sequencing and microbial functional gene array (GeoChip 5.0) to assess the above changes along a 51-year revegetation chronosequence in the Tengger Desert, China. The results showed a significant difference in AOA-community richness between 5-year-old BSCs and older ones. The most dominant phylum during BSC development was Crenarchaeota, and the corresponding species were ammonia-oxidizing_Crenarchaeote and environmental_samples_Crenarchaeota. Network analysis revealed that the positive correlations among dominant taxa increased, and their cooperation was reinforced in AOA communities during BSC succession. Redundancy analysis showed that the dominant factor influencing the change in AOA-community structure was soil texture. GeoChip 5.0 indicated that the amoA gene abundances of AOA and ammonia-oxidizing bacteria (AOB) were basically the same, demonstrating that AOA and AOB played an equally important role during BSCs development. Our study of the long-term succession of BSC demonstrated a persistent response of AOA communities to revegetation development in desert ecosystems.

Key words: ammonia-oxidizing archaea, biological soil crusts, GeoChip 5.0, network analysis

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Soil characteristics 5YR 15YR 28YR 34YR 51YR
pH 8.22±0.07 8.24±0.08 8.20±0.03 8.25±0.02 8.09±0.05
Sand (%) 99.10±0.12 93.88±0.71 93.51±1.27 90.16±1.47 85.74±1.24
Silt (%) 0.76±0.14 5.86±0.75 6.09±1.31 9.32±1.53 13.64±1.22
Clay (%) 0.14±0.03 0.26±0.05 0.40±0.05 0.52±0.06 0.62±0.06
Organic Matter (g/kg) 0.54±0.03 2.10±0.18 1.67±0.63 2.79±0.52 2.93±0.26
Carbon: Nitrogen ratio 2.54±1.16 4.51±0.35 4.12±0.17 4.66±0.25 4.68±0.40
Total Carbon (g/kg) 0.45±0.07 3.53±0.61 3.08±2.03 6.53±1.15 7.23±0.15
Total Nitrogen (g/kg) 0.14±0.05 0.27±0.01 0.24±0.09 0.35±0.07 0.36±0.01
Total Phosphorus (g/kg) 0.28±0.03 0.49±0.03 0.48±0.13 0.62±0.09 0.63±0.03
Available Nitrogen (mg/kg) 10.16±1.80 17.55±2.17 21.35±3.31 26.99±5.77 29.96±14.83
Available Phosphorus (mg/kg) 1.30±0.48 7.22±0.30 4.70±3.21 7.41±2.47 6.68±1.40
Urease (μg N-NH 4 + /(g?h)) 0.79±0.00 3.85±0.25 3.96±0.04 6.80±0.04 10.46±0.16

Dehydrogenase

(μg triphenyl formazan/(g?h))

 0.64±0.02 0.71±0.02 0.74±0.02 0.76±0.02 0.90±0.01
Invertase (μg glucose/(g?h)) 11.61±3.26 54.27±1.56 44.33±12.75 39.62±8.40 38.33±3.39

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Sample ID Sequences numbers Mean length OTU
5YR1 14,959 272.668828 26
5YR2 13,765 271.275699 23
5YR3 17,437 272.507140 26
Even 15,387 272.150556 25
15YR1 12,705 271.997403 40
15YR2 16,331 271.610618 45
15YR3 18,192 274.208828 39
Even 15,743 272.605616 41
28YR1 12,032 270.735206 38
28YR2 17,346 271.312810 29
28YR3 16,824 272.688421 32
Even 15,401 271.578812 33
34YR1 17,985 274.157687 43
34YR2 17,306 271.427135 37
34YR3 10,409 271.250841 37
Even 15,233 272.278554 39
51YR1 18,602 272.207773 36
51YR2 12,914 270.748335 40
51YR3 10,190 271.624436 38
Even 13,902 271.526848 38

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Attributes IT DHA UE AP AN TP TN TC C:N OM Clay Silt Sand pH
pH 0.032 -0.629* -0.548* -0.175 -0.366 -0.31 -0.426 -0.382 0.024 -0.313 -0.459 -0.526* 0.525* 1
Sand -0.397 -0.925** -0.972** -0.634* -0.742** -0.838** -0.819** -0.917** -0.726** -0.885** -0.896** -1.000** 1
Silt 0.395 0.922** 0.970** 0.634* 0.735** 0.836** 0.818** 0.916** 0.725** 0.884** 0.888** 1
Clay 0.404 0.901** 0.932** 0.559* 0.818** 0.789** 0.765** 0.857** 0.665** 0.827** 1
OM 0.572* 0.759** 0.858** 0.884** 0.773** 0.965** 0.953** 0.971** 0.784** 1
C:N 0.675** 0.586* 0.675** 0.702** 0.659** 0.711** 0.573* 0.703** 1
TC 0.415 0.801** 0.901** 0.796** 0.730** 0.956** 0.946** 1
TN 0.488 0.719** 0.800** 0.863** 0.687** 0.955** 1
TP 0.495 0.717** 0.803** 0.899** 0.734** 1
AN 0.342 0.701** 0.732** 0.686** 1
AP 0.599* 0.483 0.586* 1
UE 0.364 0.962** 1
DHA 0.373 1
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