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Sciences in Cold and Arid Regions ›› 2017, Vol. 9 ›› Issue (1): 89–96.doi: 10.3724/SP.J.1226.2017.00089

• • 上一篇    

Identification of Kalidium species (Chenopodiaceae) by DNA barcoding

XiaoHui Liang, YuXia Wu   

  1. State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China
  • 收稿日期:2016-07-12 修回日期:2016-10-19 发布日期:2018-11-23
  • 通讯作者: YuXia Wu, School of Life Sciences, Lanzhou University. No. 222, South Tianshui Road, Lanzhou, Gansu 730000, China. E-mail:wuyx@lzu.edu.cn E-mail:YuXia Wu, School of Life Sciences, Lanzhou University. No. 222, South Tianshui Road, Lanzhou, Gansu 730000, China. E-mail:wuyx@lzu.edu.cn

Identification of Kalidium species (Chenopodiaceae) by DNA barcoding

XiaoHui Liang, YuXia Wu   

  1. State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China
  • Received:2016-07-12 Revised:2016-10-19 Published:2018-11-23
  • Contact: YuXia Wu, School of Life Sciences, Lanzhou University. No. 222, South Tianshui Road, Lanzhou, Gansu 730000, China. E-mail:wuyx@lzu.edu.cn E-mail:YuXia Wu, School of Life Sciences, Lanzhou University. No. 222, South Tianshui Road, Lanzhou, Gansu 730000, China. E-mail:wuyx@lzu.edu.cn

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摘要： DNA barcoding is an increasingly prevalent molecular biological technology which uses a short and conserved DNA fragment to facilitate rapid and accurate species identification. Kalidium species are distributed in saline soil habitat throughout Southeast Europe and Northwest Asia, and used mainly as forage grass in China. The discrimination of Kalidium species was based only on morphology-based identification systems and limited to recognized species. Here, we tested four DNA candidate loci, one nuclear locus (ITS, internal transcribed spacer) and three plastid loci (rbcL, matK and ycf1b), to select potential DNA barcodes for identifying different Kalidium species. Results showed that the best DNA barcode was ITS locus, which displayed the highest species discrimination rate (100%), followed by matK (33.3%), ycf1b (16.7%), and rbcL (16.7%). Meanwhile, four loci clearly identified the variant species, Kalidium cuspidatum (Ung.-Sternb.) Grub.var. sinicum A. J. Li, as a single species in Kalidium.

关键词: DNA barcoding, Kalidium, species identification

Abstract: DNA barcoding is an increasingly prevalent molecular biological technology which uses a short and conserved DNA fragment to facilitate rapid and accurate species identification. Kalidium species are distributed in saline soil habitat throughout Southeast Europe and Northwest Asia, and used mainly as forage grass in China. The discrimination of Kalidium species was based only on morphology-based identification systems and limited to recognized species. Here, we tested four DNA candidate loci, one nuclear locus (ITS, internal transcribed spacer) and three plastid loci (rbcL, matK and ycf1b), to select potential DNA barcodes for identifying different Kalidium species. Results showed that the best DNA barcode was ITS locus, which displayed the highest species discrimination rate (100%), followed by matK (33.3%), ycf1b (16.7%), and rbcL (16.7%). Meanwhile, four loci clearly identified the variant species, Kalidium cuspidatum (Ung.-Sternb.) Grub.var. sinicum A. J. Li, as a single species in Kalidium.

Key words: DNA barcoding, Kalidium, species identification

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