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Sciences in Cold and Arid Regions ›› 2017, Vol. 9 ›› Issue (5): 503-510.doi: 10.3724/SP.J.1226.2017.00503

• ARTICLES • Previous Articles    

High-quality draft genome sequence of Streptomyces agglomeratus 5-1-8 with strong anti-MRSA ability, isolated from the frozen soil of Tibet in China

XiMing Chen1,2, Ling Zhang3, HaiLi Sun4, ShuYan Li5, SiJing Chang1,2, QingFeng Zhang6, BingLin Zhang1,2, Tuo Chen1,2, GuangXiu Liu1,2, Paul Dyson7   

  1. 1. Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China;
    2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou, Gansu 730000, China;
    3. Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, Xinjiang 830011, China;
    4. School of Chemistry and Environment Science, Lanzhou City University;Lanzhou, Gansu 730000, China;
    5. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China;
    6. School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China;
    7. Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK
  • Received:2016-10-19 Revised:2017-01-13 Published:2018-11-23
  • Contact: ShiChang Kang,liugx@lzb.ac.cn E-mail:liugx@lzb.ac.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (Grant No. 31400437), the international cooperation program of Gansu (1504WKCA097), the application transformation foundation of CAS (HHS-CGZH-16-02), and UK BBSRC China Partnering Grant (BB/J020419/1).

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Abstract: Streptomyces agglomeratus 5-1-8 with strong anti methicillin-resistant Staphylococcus aureus (MRSA) ability, isolated from the frozen soil of Tibet in China, has a strong ability to kill the multi-drugs-resistant MRSA. To identify the second-ary metabolism ability of this strain, we describe here the phenotypic characteristics of this strain, along with its high-quality draft genome sequence, its annotation, and analysis. The 7.1M draft genome encodes 6,284 putative open reading frames (ORFs), of which 4,416 ORFs were assigned with clusters of orthologous genes (COG) categories. Also, 65 tRNA genes and 24 rRNA operons were identified. The genome contains 12 gene clusters involved in antibiotics production and 1 gene cluster involved in anticancer-compounds production; 4 gene clusters belong to polyketides and nonribosomal peptides, 1 gene cluster belong to the butyrolactone, 4 gene clusters belong to the bacteriocin or lantipeptide, and 3 gene clusters belong to the others. This genome-sequence data will facilitate efforts to probe the potential of new antibiotics to kill multi-drugs-resistant MRSA.

Key words: Streptomyces, antibiotic, multi-drugs-resistant MRSA, genome, gene cluster

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