Sciences in Cold and Arid Regions ›› 2016, Vol. 8 ›› Issue (1): 65-71.doi: 10.3724/SP.J.1226.2016.00065

• ARTICLES • Previous Articles    

Effects on antioxidant enzyme activities and osmolytes in Halocnemum strobilaceum under salt stress

TianPeng Gao1,3, Rui Guo1, XiangWen Fang2, ZhiGang Zhao2, GuoHua Chang1,3, YinQuan Chen1,3, Qing Zhang1   

  1. 1. Centre of Urban Ecology and Environmental Biotechnology, Lanzhou City University, Lanzhou, Gansu 730070, China;
    2. State Key Laboratory of Grassland Agro-ecosystems, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China;
    3. School of Chemistry and Environment Sciences, Lanzhou City University, Lanzhou, Gansu 730070, China
  • Received:2015-06-15 Revised:2015-09-07 Published:2018-11-23
  • Contact: TianPeng Gao
  • Supported by:
    This work was funded by the National Natural Science Foundation of China (Grant Nos. 31160118, 31460162, 31422011, 31370402, and 30800122).

Abstract: The seedlings of Halocnermum strobilaceum were cultivated in 0.5% hoagland nutrient solution containing 0.0%, 0.9%, 2.7% and 5.4% of NaCl as well as composite salt (Na+, Ca2+, K+, Si4+) for 20 days; all the contents are in weight ratio. Succulent level, inorganic ions (Na+, K+), organics such as betaine, proline, malondialdehyde, and antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), betaine aldehyde dehydrogenase (BADH) were measured to reveal its salt tolerance mechanism. When the composite salt concentration reaches 5.4%, SOD activity level, and MDA content is five times the control group; when it reaches 2.7%, the succulent level of seedlings, and the content of K+ in roots is nearly two times the NaCl treatment; the dry weight is more than three times the control group; with the NaCl treatment, MDA is three times the contrast; when the salt concentration is 2.7%, POD reaches the maximum. Results indicate that Si4+, K+, and Ca2+ from composite salt in the roots of H. strobilaceum improved the water-holding capacity. The activities of antioxidant enzyme were raised by the accumulation of proline and betaine, which increased the salt tolerance. The absorption of K+ promoted the high ratio of K+/Na+ and alleviated the damage of cell membranes of H. strobilaceum, which is associated with osmotic contents such as betaine and proline.

Key words: Halocnermum strobilaceum (Pall.), salt stress, succulent, antioxidant enzyme activities

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